製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Frizzled 8
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    SYPKQMPLSQV

    , corresponding to C terminal amino acids 684-694 of Human Frizzled 8

  • ポジティブ・コントロール
    • Human pancreas and heart lysate This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: Hek293

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).
IHC-P Use a concentration of 3 µg/ml.

ターゲット情報

  • 機能
    Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
  • 組織特異性
    Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
  • 配列類似性
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • ドメイン
    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 翻訳後修飾
    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • 細胞内局在
    Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Frizzled 8 seven transmembrane spanning receptor antibody
    • frizzled 8, seven transmembrane spanning receptor antibody
    • Frizzled family receptor 8 antibody
    • frizzled homolog 8 (Drosophila) antibody
    • Frizzled homolog 8 antibody
    • Frizzled-8 antibody
    • FZ 8 antibody
    • Fz-8 antibody
    • FZ8 antibody
    • FZD 8 antibody
    • FZD8 antibody
    • FZD8_HUMAN antibody
    • hFZ 8 antibody
    • hFz8 antibody
    • HGNC4046 antibody
    • Homolog of Drosophila Frizzled 8 antibody
    see all

画像

  • ICC/IF image of ab40012 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40012 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Frizzled 8 antibody (ab40012) at 1 µg/ml + Human Pancreas lysate in RIPA buffer at 35 µg

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab40012 at 3µg/ml staining Frizzled 8 in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

参考文献

This product has been referenced in:
  • Gammons MV  et al. Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay. J Cell Sci 129:3892-3902 (2016). Human . Read more (PubMed: 27744318) »

See 1 Publication for this product

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