製品の概要

  • 製品名Anti-Frizzled 7 antibody
    Frizzled 7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Frizzled 7
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Dog
  • 免疫原

    A region within synthetic peptide: PDFTVFMIKY LMTMIVGITT GFWIWSGKTL QSWRRFYHRL SHSSKGETAV, corresponding to amino acids 525-574 of Frizzled 7

  • ポジティブ・コントロール
    • HepG2 cell lysate, Human kidney This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab51049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 組織特異性High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
  • 配列類似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • ドメインLys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Frizzled 7, seven transmembrane spanning receptor antibody
    • frizzled class receptor 7 antibody
    • Frizzled drosophila homolog of 7 antibody
    • Frizzled family receptor 7 antibody
    • Frizzled homolog 7 antibody
    • Frizzled-7 antibody
    • Frizzled7 antibody
    • Fz 7 antibody
    • Fz-7 antibody
    • Fz7 antibody
    • FZD 7 antibody
    • FZD7 antibody
    • FZD7_HUMAN antibody
    • FzE 3 antibody
    • FzE3 antibody
    • hFz 7 antibody
    • hFz7 antibody
    • Homolog of Drosophila Frizzled 7 antibody
    • OTTHUMP00000163742 antibody
    see all

Anti-Frizzled 7 antibody 画像

  • ICC/IF image of ab51049 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51049 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1 : Marker
    Lane 2 : Anti-Frizzled 7 antibody (ab51049) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Gel concentration: 12%
  • ab51049 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded; magnification 400x.ab51049 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded; magnification 400x.
  • Lane 2 : Anti-Frizzled 7 antibody (ab51049) at 2 µg/ml
    Lane 3 : Anti-Frizzled 7 antibody (ab51049) at 1 µg/ml
    Lane 4 : Anti-Frizzled 7 antibody (ab51049) at 0.5 µg/ml
    Lane 5 : Anti-Frizzled 7 antibody (ab51049) at 0.25 µg/ml

    Lane 2 : Cell lysate prepared from HepG2 cells
    Lane 3 : Cell lysate prepared from HepG2 cells
    Lane 4 : Cell lysate prepared from HepG2 cells
    Lane 5 : Cell lysate prepared from HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 64 kDa

Anti-Frizzled 7 antibody (ab51049) 使用論文

This product has been referenced in:
  • McIver SC  et al. A unique combination of male germ cell miRNAs coordinates gonocyte differentiation. PLoS One 7:e35553 (2012). WB ; Mouse . Read more (PubMed: 22536405) »
  • Gökmen-Polar Y  et al. Dual targeting of EphA2 and ER restores tamoxifen sensitivity in ER/EphA2-positive breast cancer. Breast Cancer Res Treat 127:375-84 (2011). WB ; Human . Read more (PubMed: 20602165) »

See all 3 Publications for this product

Product Wall



The Anti-Frizzled 7 antibody (ab51049) could be used with the following secondary antibodies in Western blotting:

Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), ab16284

Goat polyclonal Secondary Antibody...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"