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RYATLEHPFHCPR, corresponding to internal sequence amino acids 191-203 of Human Frizzled 2
Our Abpromise guarantee covers the use of ab109094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 63.6 kDa). Preliminary experiments gave an approx. 23kDa band in Human Colon, Heart and Ovary lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kDa according to NP_001457.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide.|
|ICC/IF||Use a concentration of 10 µg/ml.|
ICC/IF image of ab109094 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells. The cells were then incubated with the antibody ab109094 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab109094 (2.5ug/ml) staining frizzled 2 in paraffin-embedded human thyroid tissue sections by Immunohistochemistry
ab109094 has not yet been referenced specifically in any publications.
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