製品の概要

  • 製品名Anti-Frizzled 10 antibody
    Frizzled 10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Frizzled 10
  • アプリケーション適用あり: IHC-FoFr, WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Horse, Chicken, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (PIEIPMCKDI GYNMTRMPNL MGHENQREAA IQLHEFAPLV EYGCHGHLRF) of Human Frizzled 10 (NP_009128).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 23251385
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

ターゲット情報

  • 機能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 組織特異性Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
  • 配列類似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • ドメインLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 翻訳後修飾Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 350 antibody
    • CD 350 antigen antibody
    • CD350 antibody
    • Frizzled (Drosophila) homolog 10 antibody
    • Frizzled-10 antibody
    • FZ 10 antibody
    • Fz-10 antibody
    • FZ10 antibody
    • FZD 10 antibody
    • FZD10 antibody
    • FZD10_HUMAN antibody
    • FzE 7 antibody
    • FzE7 antibody
    • hFz 10 antibody
    • hFz10 antibody
    see all

Anti-Frizzled 10 antibody 画像

  • Immunohistochemical image of ab83044 (dilution 1/1000) staining in rat spinal cord PFA fixed frozen tissue section, performed on an Olympus BX61 Motorized Research Microscope. The scale bar represents 50 µm.

  • Anti-Frizzled 10 antibody (ab83044) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 12%

Anti-Frizzled 10 antibody (ab83044) 使用論文

This product has been referenced in:
  • Gonzalez P  et al. Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats. PLoS One 7:e50793 (2012). IHC-P ; Rat . Read more (PubMed: 23251385) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"