製品の概要

  • 製品名Anti-Frizzled 10 antibody
    Frizzled 10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Frizzled 10
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis, Chimpanzee, Monkey
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide:

    A PNNGSDEPTR GSGLFPPLFR PQRPHSAQEH PLKDGGPGRG GCDNPGKFHH

    , corresponding to amino acids 150-200 of Human Frizzled 10. (NP_009128).

  • ポジティブ・コントロール
    • Human brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

ターゲット情報

  • 機能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 組織特異性Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
  • 配列類似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • ドメインLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 翻訳後修飾Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 350 antibody
    • CD 350 antigen antibody
    • CD350 antibody
    • Frizzled (Drosophila) homolog 10 antibody
    • Frizzled-10 antibody
    • FZ 10 antibody
    • Fz-10 antibody
    • FZ10 antibody
    • FZD 10 antibody
    • FZD10 antibody
    • FZD10_HUMAN antibody
    • FzE 7 antibody
    • FzE7 antibody
    • hFz 10 antibody
    • hFz10 antibody
    see all

Anti-Frizzled 10 antibody 画像

  • Lane 1 : Pre-immune serum
    Lane 2 : Anti-Frizzled 10 antibody (ab71987) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate


    Predicted band size : 65 kDa
    Observed band size : 65 kDa

Anti-Frizzled 10 antibody (ab71987) 使用論文

ab71987 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (Eye)
Loading amount 15 µg
Specification Eye
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Nov 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"