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Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 (MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK) of Human Frequenin (NP_055101).
Our Abpromise guarantee covers the use of ab105524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling Frequenin with ab105524 at 4-8µg/ml. Arrows indicate positively labelled neural cells. Magnification: 400X.
ab105524 has not yet been referenced specifically in any publications.