Anti-FREM2 抗体 (ab75803)

製品の概要

  • 製品名Anti-FREM2 antibody
    FREM2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FREM2
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human FREM2.

    (Peptide available as ab95841.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: EBS E14 TG2A Day 3; EBS E14 TG2A Day 5; EBS E14 TG2A Day 8.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 351 kDa (predicted molecular weight: 351 kDa). Also detects a band of approximately 296 kDa, which corresponds to the predicted molecular weight of isoform 2.

ターゲット情報

  • 機能Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion.
  • 関連疾患Defects in FREM2 are a cause of Fraser syndrome (FRASS) [MIM:219000]. Fraser syndrome is a multisystem malformation usually comprising cryptophthalmos, cutaneous syndactyly, ear abnormalities, renal agenesis and congenital heart defects.
  • 配列類似性Belongs to the FRAS1 family.
    Contains 5 Calx-beta domains.
    Contains 12 CSPG (NG2) repeats.
  • ドメインThe Calx-beta domains bind calcium with high affinity and undergo a major conformational shift upon binding.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781I048 antibody
    • ECM3 homolog antibody
    • FRAS1-related extracellular matrix protein 2 antibody
    • FREM 2 antibody
    • Frem2 antibody
    • FREM2_HUMAN antibody
    • KIAA1074 antibody
    see all

Anti-FREM2 antibody 画像

  • All lanes : Anti-FREM2 antibody (ab75803) at 1 µg/ml

    Lane 1 : EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 2 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 3 : EBS E14 TG2A Day 8 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 351 kDa
    Observed band size : 351 kDa
    Additional bands at : 100 kDa,296 kDa (possible isoform),45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab75803 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FREM2 antibody (ab75803) 使用論文

ab75803 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK-293 cells)
Specification HEK-293 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Feb 18 2014

Thank you for contacting us. We have not tested ab75803 in Flow Cytometry and do not have any data regarding suitability in this application. You would have to purify the antibody before conjugating it to remove the BSA or the BSA may be conjugated. Read More

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (EpiSC)
Specification EpiSC
Fixative Paraformaldehyde
Permeabilization Yes - 0.1 triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Abcam user community

Verified customer

投稿 Nov 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"