製品の概要

  • 製品名Anti-Fragilis antibody
    Fragilis 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fragilis
  • アプリケーション適用あり: IP, IHC-P, IHC-G, ICC/IF, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from residues 1 - 50 of Mouse Fragilis.

    (Peptide available as ab15737.)

  • ポジティブ・コントロール
    • WB: Mouse Embryonic Stem Cell lysate, Mouse Embryonic Germ Cell lysate IHC-Fr: male genital ridges of E12.5 mouse embryos

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21615296
IHC-G Use at an assay dependent concentration. PubMed: 22446628
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).

ターゲット情報

  • 機能IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.
  • 配列類似性Belongs to the CD225 family.
  • 翻訳後修飾Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 8U antibody
    • Fragilis antibody
    • IFITM3 antibody
    • IFM3_HUMAN antibody
    • Interferon induced transmembrane protein 3 (1 8U) antibody
    • Interferon Induced Transmembrane Protein 3 antibody
    • Interferon inducible antibody
    • Interferon inducible protein 1 8U antibody
    • Interferon Inducible Protein 15 antibody
    • Interferon Inducible Protein Homolog antibody
    • Interferon-induced transmembrane protein 3 antibody
    • Interferon-inducible protein 1-8U antibody
    • IP15 antibody
    see all

Anti-Fragilis antibody 画像

  • IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).

    ab15592 (anti-Fragilis) = red
    Oct4 = green
    DAPI (DNA) = blue

    The dilution of ab15592 used was 10 ug/ml.

  • Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
  • Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 13kDa: Fragilis - Primordial Germ Cell Marker.

Anti-Fragilis antibody (ab15592) 使用論文

This product has been referenced in:
  • Zhou L  et al. Production of fat-1 transgenic rats using a post-natal female germline stem cell line. Mol Hum Reprod 20:271-81 (2014). Rat . Read more (PubMed: 24258451) »
  • Wakim LM  et al. Enhanced survival of lung tissue-resident memory CD8? T cells during infection with influenza virus due to selective expression of IFITM3. Nat Immunol 14:238-45 (2013). WB . Read more (PubMed: 23354485) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis of E14.5 mouse embryos)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-based solution
Permeabilization Yes - 0.2% gelatin, 0.25% Triton X-100 in PBS 1×
Specification Testis of E14.5 mouse embryos
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Mr. Sami Zaqout

Verified customer

投稿 Oct 03 2016

He llevado a cabo la alineación de secuencias de la secuencia que me has enviado con la correspondiente al inmunógeno de ab74699 y resulta un porcentaje del 66%:

Sequence type explicitly set to Protein

Sequence format ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Apr 13 2012

Thank you for your enquiry. I have looked at the amino acid sequence used to generate the antibody and can confirm it is at the N terminus of the protein, however the SwissProt reference we currently have (Q9D8L6) does not specify which part of the pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"