製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FPRL1
  • 特異性
    This antibody recognizes human FPRL-1.
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues of 2nd extracellular loop of human FPRL1, KLH conjugated.

  • ポジティブ・コントロール
    • HL60 cells and extracts from HL60 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).
Flow Cyt 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

ターゲット情報

  • 機能
    Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
  • 組織特異性
    Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALXR antibody
    • FMLP-R-I antibody
    • FMLP-R-II antibody
    • FMLP-related receptor I antibody
    • FMLPX antibody
    • Formyl peptide receptor 2 antibody
    • Formyl peptide receptor related antibody
    • Formyl peptide receptor-like 1 antibody
    • FPR2 antibody
    • FPR2_HUMAN antibody
    • FPR2A antibody
    • FPRH1 antibody
    • FPRH2 antibody
    • FPRL1 antibody
    • HM63 antibody
    • Lipoxin A4 receptor antibody
    • LXA4 receptor antibody
    • LXA4R antibody
    • N-formyl peptide receptor 2 antibody
    • RFP antibody
    see all

画像

  • Anti-FPRL1 antibody (ab63022) at 1/5000 dilution + Extracts from HL60 cells.

    Predicted band size : 39 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent analysis of HL60 cells using ab63022 (green) at 1:500. Nucleus have bee labeled with PI (red).
  • Flow cytometric analysis of HL60 cells using ab63022 at 1:100 (red) compared to a non-specific negative control antibody (black).

参考文献

This product has been referenced in:
  • Gori I  et al. Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4). Fertil Steril 99:1965-73.e2 (2013). Read more (PubMed: 23472950) »

See 1 Publication for this product

レビューと Q&A

Thank you for your inquiry. Yes, this antibody will detect the 2nd extracellular loop of FPRL1, on the surface of cells. As for a suitable secondary antibody, we have a good goat polyclonal secondary antibody to rabbit IgG that is FITC-conjugat...

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Thank you for your inquiry. We haven't tested ab63022 or ab63023 in mouse so we do not know if these antibodies will work in mouse. I've BLASTed the immunogen sequences against mouse and the results are below: Ab63022 was immunized agai...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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