Rabbit polyclonal to FOXQ1
A region within synthetic peptide: GDDSLGSDGD CAANSPAAGG GARDPPGDGE QSAGGGPGAE EAIPAAAAAA, corresponding to N terminal amino acids 36-85 of Human FOXQ1
Jurkat cell lysate and human kidney tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Expressed predominantly in the stomach, trachea, bladder and salivary gland.
Contains 1 fork-head DNA-binding domain.
Information by UniProt
Forkhead box protein Q1 antibody
Forkhead box Q1 antibody
FOX Q1 antibody
Western blot - FOXQ1 antibody (ab51340)
Anti-FOXQ1 antibody (ab51340) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS at 5 %
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size :
Observed band size :
50 kDa (
why is the actual band size different from the predicted?
Gel concentration 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXQ1 antibody (ab51340)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXQ1 with ab51340 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells of renal tubule. Magnification: 400X.
Immunocytochemistry/ Immunofluorescence - Anti-FOXQ1 antibody (ab51340)
ICC/IF image of ab51340 stained cells. The cells were and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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