製品の概要

  • 製品名Anti-FOXP4 antibody
    FOXP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FOXP4
  • アプリケーション適用あり: IHC-FoFr, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human FOXP4.

    (Peptide available as ab20334.)

  • ポジティブ・コントロール
    • Human FOXP4 Partial Recombinant Protein (tagged) Mouse stomach tissue lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr
WB
  • 追加情報IHC-F: 1/100 - 1/300.
    WB: Use at a concentration of 1 µg/ml (Human FOXP4 Partial Recombinant Protein). Used at a dilution of 1/1000 for 12 hrs incubation on human cells (see Abreview for further details).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcriptional repressor that represses lung-specific expression.
    • 配列類似性Contains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.
    • ドメインThe leucine-zipper is required for dimerization and transcriptional repression.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1200010K03Rik antibody
      • 2310007G05Rik antibody
      • FKHLA antibody
      • FLJ40908 antibody
      • FLJ44184 antibody
      • Fork head related protein like A antibody
      • Fork head-related protein-like A antibody
      • Forkhead box P4 antibody
      • Forkhead box protein P4 antibody
      • FOX P4 antibody
      • FOXP 4 antibody
      • foxp4 antibody
      • FOXP4_HUMAN antibody
      • hFKHLA antibody
      • mFKHLA antibody
      • OTTHUMP00000016374 antibody
      • OTTHUMP00000039784 antibody
      • OTTHUMP00000043412 antibody
      • OTTHUMP00000043536 antibody
      • Winged helix repressor FOX P4 antibody
      • Winged helix repressor FOXP 4 antibody
      • Winged helix repressor FOXP4 antibody
      see all

    Anti-FOXP4 antibody 画像

    • ab17726 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) stomach. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
    • All lanes : Anti-FOXP4 antibody (ab17726) at 1/1000 dilution

      Lane 1 : 50ug Lung epithelial cell line, non-transfected.
      Lane 2 : 50ug Lung epithelial cell line, transfected with FOXP4.

      Secondary
      HRP cpnjugated goat anti-rabbit

      Performed under reducing conditions.

      Predicted band size : 11 kDa


      Exposure time : 1 second

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Anti-FOXP4 antibody (ab17726) at 1 µg/ml + Human FOXP4 Partial Recombinant Protein (Tagged) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 11 kDa
      Observed band size : 11 kDa

    Anti-FOXP4 antibody (ab17726) 使用論文

    ab17726 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (lung epithelial cell line)
    Loading amount 50 µg
    Specification lung epithelial cell line
    Treatment transfected
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
    Username

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    Verified customer

    投稿 Jul 06 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"