Anti-FOXP3 抗体 [mAbcam 22511] (ab22511)

製品の概要

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab22511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
IHC-Fr
WB
  • 追加情報IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Can be blocked with FOXP3 peptide (ab16809).

    Tested in Western blotting against human FOXP3 transfectants of 293 cells.

    This clone has been tested against FOXP3 transfectants of COS-1 cells fixed with acetone and also works against formalin-fixed paraffin-embedded FOXP3 transfectants heat retrieved with pH9 Tris-EDTA buffer. It did not show positive staining with cryostat or paraffin-embedded human tonsil.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Probable transcription factor. Plays a critical role in the control of immune response.
    • 関連疾患Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
    • 配列類似性Contains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    Anti-FOXP3 antibody [mAbcam 22511] 画像

    • All lanes : Anti-FOXP3 antibody [mAbcam 22511] (ab22511) at 1 µg/ml

      Lane 1 : HEK293 cells overexpressing human FOXP3
      Lane 2 : HEK293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 48 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 45 seconds
    • Clone mAbcam 22511 used in immunocytochemistry on transfected COS-1 cytospins fixed with acetone for 10 mins.

      1: FOXP1 transfectant
      2: FOXP2 transfectant
      3: FOXP3 transfectant
      4: FOXP4 transfectant

      Staining can be seen on the FOXP3 transfectant, but no staining is seen with the other FOXP protein transfectants, confirming the specificity of this antibody in immunocytochemistry.

      These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

    • Clone mAbcam 22511 used in immunocytochemistry on paraffin embedded transfected COS-1 cytospins.

      1: FOXP1 transfectant
      2: FOXP2 transfectant
      3: FOXP3 transfectant
      4: FOXP4 transfectant

      Strong staining can be seen on the FOXP3 transfectant, but no staining is seen with the other FOXP protein transfectants. This shows that the antibody is specific for FOXP3 when used with paraffin embedded cells.

      These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

    Anti-FOXP3 antibody [mAbcam 22511] (ab22511) 使用論文

    ab22511 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (tonsil)
    Specification tonsil
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Apr 07 2009

    Thank you for your enquiry. ab450, ab20034, ab22510, ab22511 have all been tested and shown to work on formalin fixed tissue. However, may I recommend the monoclonal, ab22510 FOXP3 antibody [mAbcam 22510]. This has been used in the following public...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"