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Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human FOXP1.
(Peptide available as ab20276.)
Our Abpromise guarantee covers the use of ab16645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/200 - 1/500. PubMed: 20364144|
|IP||Use at an assay dependent concentration.|
|WB||1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details).
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Image courtesy of Human Protein Atlas
ab16645 staining FOXP1 in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab16645 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab16645 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org