製品の概要

  • 製品名Anti-Foxo6 antibody
    Foxo6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Foxo6
  • 特異性This antibody recognizes Foxo6
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide: RAVSMDNGAK FLRIKGKASK KKQLHLPERS PDDSPPGAPV PGPLSASAKW, corresponding to a region within N terminal amino acids 181-230 of Mouse Foxo6

  • ポジティブ・コントロール
    • WB: SP2/0 cell lysate. ICC/IF: MEF1 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性Murine Foxo6 is a member of the murine forkhead family of transcription factors. This family consists of over 30 members, the vast majority of which is important in embryonic development. These forkhead transcription factors may play a role in maintenance and survival of developing and adult neurons.
  • 細胞内局在Cytoplasmic and Nuclear. When phosphorylated, translocated from nucleus to cytoplasm. High nuclear localization after stimulation with growth factors.
  • 参照データベース
  • 別名
    • Forkhead box protein O6 antibody

Anti-Foxo6 antibody 画像

  • Anti-Foxo6 antibody (ab48730) at 1.25 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab48730 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Foxo6 antibody (ab48730) 使用論文

ab48730 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"