製品の概要

  • 製品名Anti-FOXO3A antibody [EPR1950]
    FOXO3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1950] to FOXO3A
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human FOXO3A.

  • ポジティブ・コントロール
    • MCF7 and SH-SY5Y cell lysates, HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 71 kDa).
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
    • 組織特異性Ubiquitous.
    • 関連疾患Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
    • 配列類似性Contains 1 fork-head DNA-binding domain.
    • 翻訳後修飾In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
    • 細胞内局在Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
    • Information by UniProt
    • 参照データベース
    • 別名
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      • Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
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      • Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
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      • Forkhead in rhabdomyosarcoma like 1 antibody
      • Forkhead in rhabdomyosarcoma-like 1 antibody
      • FOX O3A antibody
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      • foxo3 antibody
      • FOXO3_HUMAN antibody
      • FOXO3A antibody
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    Anti-FOXO3A antibody [EPR1950] 画像

    • Immunofluorescent staining of HeLa cells using ab109629 at a dilution of 1/50.
    • All lanes : Anti-FOXO3A antibody [EPR1950] (ab109629) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 71 kDa
    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO3A with unpurified ab109629 at 1/150 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    Anti-FOXO3A antibody [EPR1950] (ab109629) 使用論文

    This product has been referenced in:
    • Hu T  et al. Reprogramming ovarian and breast cancer cells into non-cancerous cells by low-dose metformin or SN-38 through FOXO3 activation. Sci Rep 4:5810 (2014). WB, ICC/IF ; Human . Read more (PubMed: 25056111) »

    See 1 Publication for this product

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