製品の概要

  • 製品名Anti-FOXO3A antibody [3C2]
    FOXO3A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3C2] to FOXO3A
  • アプリケーション適用あり: WB, IHC-P, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Pig
  • 免疫原

    Recombinant fragment: PCTVELPRLT DMAGTMNLND GLTENLMDDL LDNITLPPSQ PSPTGGLMQR SSSFPYTTKG SGLGSPTSSF NSTVFGPSSL NSLRQSPMQT IQENKPATFS, corresponding to amino acids 361-460 of Human FOXO3A with a proprietary tag (AAH21224).

  • ポジティブ・コントロール
    • Human spleen tissue; Recombinant tagged FOXO3A

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab124394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
  • 組織特異性Ubiquitous.
  • 関連疾患Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 翻訳後修飾In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
  • 細胞内局在Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
  • Information by UniProt
  • 参照データベース
  • 別名
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    • Forkhead box protein O3 antibody
    • Forkhead box protein O3A antibody
    • Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
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    • Forkhead in rhabdomyosarcoma like 1 antibody
    • Forkhead in rhabdomyosarcoma-like 1 antibody
    • FOX O3A antibody
    • FOXO2 antibody
    • foxo3 antibody
    • FOXO3_HUMAN antibody
    • FOXO3A antibody
    • MGC12739 antibody
    • MGC31925 antibody
    see all

Anti-FOXO3A antibody [3C2] 画像

  • ab124394, at 5 µg/ml, staining FOXO3A in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry.
  • Detection limit for recombinant tagged FOXO3A is approximately 0.3ng/ml as a capture antibody.

Anti-FOXO3A antibody [3C2] (ab124394) 使用論文

ab124394 has not yet been referenced specifically in any publications.

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