Anti-FOXO1A (phospho S256) 抗体 (ab131339)
Key features and details
- Rabbit polyclonal to FOXO1A (phospho S256)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXO1A (phospho S256) antibody
FOXO1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXO1A (phospho S256) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human FOXO1A conjugated to keyhole limpet haemocyanin. Synthetic peptide corresponding to the sequence around phosphorylation site of serine 256 (
A-A-S(p)-M-D
) of Human FOXO1A (NP_002006.2).
Sequence:A-A-S(p)-M-D
Database link: Q12778 -
ポジティブ・コントロール
- MCF7 cells, 293 cell extracts, Human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.88% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab131339 is purified by affinity-chromatography using an epitode-specific phosphopetide. Non-phoso specific antibodies are removed by chromatography using a non-phosphopeptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131339の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 78-82 kDa (predicted molecular weight: 69 kDa). Incubate membrane with diluted antibody in 5% non fatmilk, 1X TBS, 0.1% Tween 20 at 4 C, with gentle shaking, overnight.
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF | (1) |
1/100 - 1/200.
|
特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 78-82 kDa (predicted molecular weight: 69 kDa). Incubate membrane with diluted antibody in 5% non fatmilk, 1X TBS, 0.1% Tween 20 at 4 C, with gentle shaking, overnight. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. -
組織特異性
Ubiquitous. -
関連疾患
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
翻訳後修飾
Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2308 Human
- Omim: 136533 Human
- SwissProt: Q12778 Human
- Unigene: 370666 Human
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別名
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
see all
画像
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Immunofluorescent analysis of formalin-fixed, paraffin-embedded human breast tissue with Anti-FOXO1A at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.
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All lanes : Anti-FOXO1A (phospho S256) antibody (ab131339) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts treated with serum
Predicted band size: 69 kDa -
Immunofluorescence analysis of methanol-fixed MCF7 cells using ab131339 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab131339 at a 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab131339 は 35 報の論文で使用されています。
- Peng M et al. Programmed death-ligand 1 signaling and expression are reversible by lycopene via PI3K/AKT and Raf/MEK/ERK pathways in tongue squamous cell carcinoma. Genes Nutr 17:3 (2022). PubMed: 35164673
- Wu B et al. Aerobic exercise promotes the expression of ATGL and attenuates inflammation to improve hepatic steatosis via lncRNA SRA. Sci Rep 12:5370 (2022). PubMed: 35354841
- Dai L et al. Propofol inhibits the malignant development of osteosarcoma U2OS cells via AMPK/FΟΧO1-mediated autophagy. Oncol Lett 24:310 (2022). PubMed: 35949604
- Ren HZ et al. FOXO1 Alleviates Liver Ischemia-reperfusion Injury by Regulating the Th17/Treg Ratio through the AKT/Stat3/FOXO1 Pathway. J Clin Transl Hepatol 10:1138-1147 (2022). PubMed: 36381102
- Zhao HY et al. L-carnitine treatment attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction. Korean J Intern Med 36:S180-S195 (2021). PubMed: 32942841