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Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319.
Our Abpromise guarantee covers the use of ab39670 in the following tested applications.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 20467422See Abreview.|
ICC/IF image of ab39670 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39670, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image is courtesy of an anonymous abreview.
Primary diluted in PBS (BSA + 0.1% Tween20) and incubated with sample for 90 minutes at 20°C.