製品の概要

  • 製品名Anti-FOXO1A antibody - ChIP Grade
    FOXO1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FOXO1A - ChIP Grade
  • 特異性ab39670 detects endogenous levels of total FOXO1A.
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, ELISA, ChIPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319.

  • ポジティブ・コントロール
    • HeLa and 293 cell extracts and human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
ChIP Use at an assay dependent concentration. PubMed: 20467422See Abreview.

ターゲット情報

  • 機能Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • 組織特異性Ubiquitous.
  • 関連疾患Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 翻訳後修飾Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Anti-FOXO1A antibody - ChIP Grade 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39670. (Left image: without peptide; Right image: with peptide 1µg/ml).
  • Lanes 1 - 3 : Anti-FOXO1A antibody - ChIP Grade (ab39670) at 1/500 dilution
    Lanes 4 - 5 : Anti-FOXO1A (phospho S319) antibody (ab38516) at 1/500 dilution

    Lane 1 : 293 Cell extract
    Lane 2 : HeLa Cell extract
    Lane 3 : HeLa Cell extract
    Lane 4 : HeLa Cell extract
    Lane 5 : HeLa Cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • ICC/IF image of ab39670 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39670, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FOXO1A antibody - ChIP Grade (ab39670) at 1/1000 dilution + Whole cell lysates prepared from human Jurkat cells at 200000 cells

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 45 kDa (possible non-specific binding),50 kDa (possible non-specific binding),80 kDa (possible non-specific binding).

    Exposure time : 30 seconds

    This image is courtesy of an anonymous abreview.

    Primary diluted in PBS (BSA + 0.1% Tween20) and incubated with sample for 90 minutes at 20°C.

Anti-FOXO1A antibody - ChIP Grade (ab39670) 使用論文

This product has been referenced in:
  • Qu X  et al. MiR-132-3p Regulates the Osteogenic Differentiation of Thoracic Ligamentum Flavum Cells by Inhibiting Multiple Osteogenesis-Related Genes. Int J Mol Sci 17:N/A (2016). WB ; Human . Read more (PubMed: 27556448) »
  • Xiao N  et al. The E3 ubiquitin ligase Itch is required for the differentiation of follicular helper T cells. Nat Immunol 15:657-66 (2014). Read more (PubMed: 24859451) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (chondrocytes)
Antigen retrieval step Other
Permeabilization No
Specification chondrocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Apr 23 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Caenorhabditis elegans Cell lysate - whole cell (whole worm lysate)
Loading amount 80 µg
Specification whole worm lysate
Gel Running Conditions Reduced Denaturing (SDS-Page 10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Lorenzo Ramos-Mucci

Verified customer

投稿 Dec 12 2012

Thank you for contacting Abcam.

I confirmed that the product sent to you was ab39670, "Anti-FOXO1A antibody - ChIP grade." This antibody is a rabbit (Rb) polyclonal (pAb) to FOXO1A, hence the name "Rb pAb to Fox01A."
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP)
Loading amount 40 µg
Specification LNCaP
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Mar 30 2012

Thank you for contacting us. Abcam does differentiate Chiq from Chip-seq; our datasheets do show these as distinct applications. However because the principal until immunoprecitation of chromatin is same so I can say the antibodies can be guarant...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - whole cell (Mouse T cells)
Specification Mouse T cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 12 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control N.A.
Negative control IP from normal rabbit Ig
Username

Dr. Weiming Ouyang

Verified customer

投稿 Jun 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 200000 cells
Specification Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 24 2009

I have received the following information from the laboratory source of those three antibodies: The antibodies have not specifically been tested against other family members of the FOX family. However, we have calculated the homology of the immunoge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"