製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FOXO1A
  • アプリケーション
    適用あり: WB, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide mapping to a region between residues 600 and the C-terminus (residue 652) of human FOXO1A (Swiss-Prot entry Q12778)

  • ポジティブ・コントロール
    • HEK293 whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/3000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP Use at 1-10 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/500.

ターゲット情報

  • 機能
    Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • 配列類似性
    Contains 1 fork-head DNA-binding domain.
  • 翻訳後修飾
    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FOXO1A with ab70382 at 1/500 (2µg/ml). Detection: DAB.
  • Lane 1 : Anti-FOXO1A antibody (ab70382) at 0.3 µg/ml
    Lane 2 : Anti-FOXO1A antibody (ab70382) at 0.1 µg/ml

    Lane 1 : Hek293 whole cell lysate
    Lane 2 : Hek293 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human FOXO1A by Immunoprecipitation. ab70382 used at 5µg/0.5mg Hek293 whole cell lysate for IP and 0.1µg/ml for WB. IP was controlled using a rabbit antibody to an unrelated protein. Detection: Chemiluminescence with exposure times of less than 2 minutes.

参考文献

This product has been referenced in:
  • Ochodnicka-Mackovicova K  et al. The DNA Damage Response Regulates RAG1/2 Expression in Pre-B Cells through ATM-FOXO1 Signaling. J Immunol 197:2918-29 (2016). Read more (PubMed: 27559048) »
  • Tobita T  et al. SIRT1 Disruption in Human Fetal Hepatocytes Leads to Increased Accumulation of Glucose and Lipids. PLoS One 11:e0149344 (2016). WB . Read more (PubMed: 26890260) »

See all 5 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ewing's sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 20min 100C
Permeabilization
No
Specification
Ewing's sarcoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
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投稿 Dec 15 2016

Application
Western blot
Sample
Pig Cell lysate - whole cell (Heart)
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 Jul 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Human Cell (human fibroblast cell)
Specification
human fibroblast cell
Permeabilization
Yes - Triton X100
Fixative
Formaldehyde
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投稿 Jan 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (human fibroblst)
Specification
human fibroblst
Total protein in input
500 µg
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投稿 Sep 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (human fibroblast)
Specification
human fibroblast
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Aug 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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