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Our Abpromise guarantee covers the use of ab1102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.1 - 2 µg/ml. Predicted molecular weight: 70 kDa.|
ab1102 staining FOXO1A in Human CD4 T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA + 0.1% Saponin) for 1 hour. An Alexa Fluor®647-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.