Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) 抗体 (ab58517)

製品の概要

  • 製品名Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody
    FOXO1 + FOXO3 + FOXO4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32)
  • 特異性ab58517 detects endogenous levels of FOXO1/3/4 only when phosphorylated at threonine 24/32.
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human FOXO1/3/4 around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).

  • ポジティブ・コントロール
    • IHC-P: human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000.

ターゲット情報

  • 関連性FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined. FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia. FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.
  • 細胞内局在FOXO1: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. FOX3: Cytoplasm, cytosol. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. FOX4: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation.
  • 参照データベース
  • 別名
    • AFX1 antibody
    • FKH1 antibody
    • FKHRL1 antibody
    • FKHRL1P2 antibody
    • Forkhead box O1 antibody
    • Forkhead box O3 antibody
    • Forkhead box O4 antibody
    • FOXO1 antibody
    • FOXO2 antibody
    • FOXO3 antibody
    • FOXO4 antibody
    • MLLT7 antibody
    see all

Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody 画像

  • This image shows human breast carcinoma tissue stained with ab58517 at a 1/50 dilution. Right hand image; with immunising phosphopeptide. Left hand image; without immunising phosphopeptide.
  • All lanes : Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)

    Lane 1 : 293 cells
    Lane 2 : 293 cells with blocking phosphopeptide

Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517) 使用論文

ab58517 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your interest in our pFOXO antibodies. None have been tested for reactivity with C. elegans, as far as we know. Here is a list of the antibodies we currently have for pFOXO:

ab47285 - FOXO3A (phospho S253)
ab38501 - FOXO1A...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"