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Our Abpromise guarantee covers the use of ab26069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 20 - 30 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human skin tissue sections, labelling FOXN1 with ab26069 at a dilution of 1/25 incubated for 18 hours at 4°C. Permeablization was with 0.05% Tween. Blocking was with 5% serum incubated for 20 minutes at room temperature. The secondary used was a swine polyclonal biotin-conjugate at 1/1000.
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