製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab26069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 20 - 30 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Transcriptional regulator involved in development.
  • 組織特異性Expressed in thymus.
  • 関連疾患Defects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND) [MIM:601705]. A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKHL20 antibody
    • Forkhead box N1 antibody
    • Forkhead box protein N1 antibody
    • FOXN 1 antibody
    • FOXN1 antibody
    • FOXN1_HUMAN antibody
    • RONU antibody
    • Rowett nude antibody
    • Transcription factor winged-helix nude antibody
    • WHN antibody
    • Winged helix nude antibody
    • Winged-helix transcription factor nude antibody
    see all

Anti-FOXN1 antibody 画像

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human skin tissue sections, labelling FOXN1 with ab26069 at a dilution of 1/25 incubated for 18 hours at 4°C. Permeablization was with 0.05% Tween. Blocking was with 5% serum incubated for 20 minutes at room temperature. The secondary used was a swine polyclonal biotin-conjugate at 1/1000.

    See Abreview

  • Ab26069 (20ug/ml) staining human FOXN1 in human thymus medulla tissue by immunohistochemistry using paraffin embedded tissue.

    Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    In paraffin embedded Human Thymus Medulla shows speculate nuclear staining in some of the Medullar cells.

Anti-FOXN1 antibody (ab26069) 使用論文

This product has been referenced in:
  • Suzuki S  et al. Transcriptional and histological analyses of the thymic developmental process in the fetal pig. Exp Anim 63:215-25 (2014). IHC ; Pig . Read more (PubMed: 24770647) »
  • Weissferdt A & Moran CA Thymic carcinoma, part 1: a clinicopathologic and immunohistochemical study of 65 cases. Am J Clin Pathol 138:103-14 (2012). IHC-P ; Human . Read more (PubMed: 22706865) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6+ tween0.05
Permeabilization Yes - tween 0.05
Specification skin
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
Username

Ms. Muriel Cario-Andre

Verified customer

投稿 Aug 19 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

啓憲 杉峰

Verified customer

投稿 Apr 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"