製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FOXM1
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 714-763 (ANRSLTEGLV LDTMNDSLSK ILLDISFPGL DEDPLGPDNI NWSQFIPELQ) of human FOXM1 (NP_068772).

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Transcriptional activatory factor. May play a role in the control of cell proliferation.
  • 組織特異性
    Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.
  • 配列類似性
    Contains 1 fork-head DNA-binding domain.
  • 発生段階
    Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus.
  • ドメイン
    Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4.
  • 翻訳後修飾
    Phosphorylated in M (mitotic) phase.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKHL16 antibody
    • Forkhead box M1 antibody
    • Forkhead box protein M1 antibody
    • forkhead like 16 antibody
    • Forkhead-related protein FKHL16 antibody
    • FOX M1 antibody
    • Foxm1 antibody
    • FOXM1_HUMAN antibody
    • FOXM1B antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
    • HFH-11 antibody
    • HFH11 antibody
    • HNF-3/fork-head homolog 11 antibody
    • HNF3 antibody
    • INS1 antibody
    • M phase phosphoprotein 2 antibody
    • M-phase phosphoprotein 2 antibody
    • MPHOSPH2 antibody
    • MPM-2 reactive phosphoprotein 2 antibody
    • MPP2 antibody
    • PIG29 antibody
    • TGT3 antibody
    • Transcription factor Trident antibody
    • Trident antibody
    • WIN antibody
    • Winged-helix factor from INS-1 cells antibody
    • Winged-helix factor from INS1 cells antibody
    see all

Anti-FOXM1 antibody 画像

  • ab83097 at 5 µg/ml staining FOXM1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-FOXM1 antibody (ab83097) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Gel concentration 12%

Anti-FOXM1 antibody (ab83097) 使用論文

This product has been referenced in:
  • Xue J  et al. Forkhead Box M1 Is Essential for Nuclear Localization of Glioma-associated Oncogene Homolog 1 in Glioblastoma Multiforme Cells by Promoting Importin-7 Expression. J Biol Chem 290:18662-70 (2015). Read more (PubMed: 26085085) »
  • Hu C  et al. LXRa-mediated downregulation of FOXM1 suppresses the proliferation of hepatocellular carcinoma cells. Oncogene N/A:N/A (2013). Read more (PubMed: 23812424) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas (Islets))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Declere
Permeabilization
No
Specification
Pancreas (Islets)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
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投稿 Apr 10 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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