1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.
Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FOXK1 with ab85999 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
Western blot - FOXK1 antibody (ab85999)
All lanes : Anti-FOXK1 antibody (ab85999) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 seconds
Immunoprecipitation - FOXK1 antibody (ab85999)
Detection of FOXK1 by Western blot of Immunprecipitate.
ab85999, at 1 µg/ml, staining FOXK1 in HeLa whole cell lysate immunoprecipitated using ab85999 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes : Anti-FOXK1 antibody (ab85999) at 1 µg/ml
Lane 1 : HeLa whole cell lysate Lane 2 : Control IgG