製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FOXJ2
  • 特異性
    ab22857 reacts with Forkhead box protein J2 (FOXJ2).
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    A region within synthetic peptide: AGASGRESAE GPPPLYNTNH DFKFSYSEIN FQDLSWSFRN LYKSMLEKSS, corresponding to internal sequence amino acids 218-267 of Human FOXJ2

  • ポジティブ・コントロール
    • HepG2 cell lysate and human stomach

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Contains 1 fork-head DNA-binding domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FHX antibody
    • Fork head homologous X antibody
    • Forkhead box J2 antibody
    • Forkhead box protein J2 antibody
    • FOXJ2 antibody
    • FOXJ2_HUMAN antibody
    see all

画像

  • Anti-FOXJ2 antibody (ab22857) at 2 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab22857, at 4 µg/ml, staining human FOXJ2 in human Stomach by Immunohistochemistry, Paraffin embedded tissue. Positive stained cells, indicated with arrows, are epithelial cells of fundic gland. Magnification 400 x.

参考文献

ab22857 has not yet been referenced specifically in any publications.

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