Anti-FOXG1 抗体 [mAbcam 51774] (ab51774)
Key features and details
- Mouse monoclonal [mAbcam 51774] to FOXG1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
-
製品名
Anti-FOXG1 antibody [mAbcam 51774]
FOXG1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [mAbcam 51774] to FOXG1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Chicken, Cow, Zebrafish -
免疫原
-
ポジティブ・コントロール
- This antibody gave a positive signal in human brain tissue lysate (ab29466).
-
特記事項
We can conjugate this antibody to FITC for you (please see ab150200 for details).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
mAbcam 51774 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51774の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use 0.5-1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
特記事項 |
---|
Flow Cyt (Intra)
Use 0.5-1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
ターゲット情報
-
機能
Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. -
組織特異性
Expression is restricted to the neurons of the developing telencephalon. -
関連疾患
Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 2290 Human
- Entrez Gene: 24370 Rat
- Omim: 164874 Human
- SwissProt: P55316 Human
- SwissProt: Q00939 Rat
- Unigene: 695962 Human
- Unigene: 708841 Human
- Unigene: 9864 Rat
-
別名
- BF-1 antibody
- BF-2 antibody
- BF1 antibody
see all
画像
-
Anti-FOXG1 antibody [mAbcam 51774] (ab51774) at 5 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands. -
Overlay histogram showing SHSY-5Y cells stained with ab51774 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51774, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab51774 は論文での使用が確認できていません。