製品の概要

  • 製品名Anti-FOXG1 antibody - ChIP Grade
    FOXG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FOXG1 - ChIP Grade
  • アプリケーション適用あり: WB, ChIP, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human FOXG1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P55316
    (Peptide available as ab19644)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human, Mouse and Rat brain tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ChIP Use at an assay dependent concentration.
IHC-P 1/1000.
IHC-Fr 1/1000.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
  • 組織特異性Expression is restricted to the neurons of the developing telencephalon.
  • 関連疾患Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BF-1 antibody
    • BF-2 antibody
    • BF1 antibody
    • BF2 antibody
    • Brain factor 1 antibody
    • Brain factor 2 antibody
    • FHKL antibody
    • FKH2 antibody
    • FKHL1 antibody
    • FKHL2 antibody
    • FKHL3 antibody
    • FKHL4 antibody
    • Forkhead box protein G1 antibody
    • Forkhead box protein G1A antibody
    • Forkhead box protein G1B antibody
    • Forkhead box protein G1C antibody
    • Forkhead like 1 antibody
    • Forkhead like 2 antibody
    • Forkhead like 3 antibody
    • Forkhead like 4 antibody
    • Forkhead-related protein FKHL1 antibody
    • Forkhead-related protein FKHL2 antibody
    • Forkhead-related protein FKHL3 antibody
    • FOXG1 antibody
    • FOXG1_HUMAN antibody
    • FOXG1A antibody
    • FOXG1B antibody
    • FOXG1C antibody
    • HBF 1 antibody
    • HBF G2 antibody
    • hBF-2 antibody
    • HBF2 antibody
    • HFK1 antibody
    • HFK2 antibody
    • HFK3 antibody
    • KHL2 antibody
    • Oncogene QIN antibody
    • QIN antibody
    see all

Anti-FOXG1 antibody - ChIP Grade 画像

  • ab18259 staining FOXG1 in Rat embryonic cortex cells overexpressing FOXG1 by ICC/IF (Immunocytochemistry/ immunofluorescence). Cells were fixed with formaldehyde and blocked with 1o% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 24 hours at 4°C. A Cy3®-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • All lanes : Anti-FOXG1 antibody - ChIP Grade (ab18259) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Human spleen tissue lysate - total protein (negative control)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 155 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-FOXG1 antibody - ChIP Grade (ab18259) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human FOXG1 peptide (ab19644) at 1 µg/ml
    Lane 5 : Brain (Mouse) Tissue Lysate with Human FOXG1 peptide (ab19644) at 1 µg/ml
    Lane 6 : Brain (Rat) Tissue Lysate with Human FOXG1 peptide (ab19644) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 1 minute

    ab18259 detects a band of ~50kDa in brain lysates. This band is blocked by addition of the immunizing peptide ab19644 which suggests that is specific for FOXG1.

     

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution.

  • ab18259 staining Foxg1 (1/50) in the telencephalon (but not the diencephalon) as expected. DAB-immunohistochemistry was performed on embryonic (E13.5) mouse brain (coronal paraffin-embedded sections) after microwave treatment with 10mM sodium citrate.

  • ab18259 staining FOXG1 in human A549 cells by immunocytochemistry/immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.25% Triton, blocked with 1% BSA for 1 hour at room temperature and then incubated with ab18259 at a 1/400 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat polyclonal used at a 1/250 dilution.

    See Abreview

Anti-FOXG1 antibody - ChIP Grade (ab18259) 使用論文

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). IF ; Human . Read more (PubMed: 26732624) »
  • Xu CC  et al. Abnormal mitochondrial transport and morphology as early pathological changes in human models of spinal muscular atrophy. Dis Model Mech 9:39-49 (2016). Human . Read more (PubMed: 26586529) »

See all 18 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cortical neurons)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification cortical neurons
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 07 2016

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (undifferentiated human iPS cells)
Specification undifferentiated human iPS cells
Blocking step 5% milk in TBST as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Feb 24 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (human neural progenitor cell from ips cell line)
Specification human neural progenitor cell from ips cell line
Permeabilization Yes - see blocking buffer
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 24 2014

After reviewing your protocol, I have some recommendations that are likely to improve your results:

1. Some epitopes are sensitive to methanol fixation, and both of the researchers in the abReviews below saw good results using a 3-4% paraforma...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (FOXG1 overexpressed rat embryonic cortex)
Specification FOXG1 overexpressed rat embryonic cortex
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 10 2013

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human fibroblast and stem cell)
Loading amount 50 µg
Specification human fibroblast and stem cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

投稿 Sep 10 2012

Thank you for submitting an Abreview of ab18259. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (E13.5 telencephalon)
Specification E13.5 telencephalon
Fixative Paraformaldehyde
Permeabilization Yes - Na citrate Antigen retrieval 70oC for 30min - PBS-T
Username

Abcam user community

Verified customer

投稿 Sep 05 2012

Vielen Dank für diese weiteren Informationen.
Es tut mir Leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456 und so...

Read More

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"