製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Probable transcription factor. Could be involved in thyroid gland organogenesis.
  • 組織特異性
    Detected in adult brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, heart, colon, small intestine testis and thymus. Expression was strongest in heart and pancreas.
  • 関連疾患
    Defects in FOXE1 are the cause of Bamforth-Lazarus syndrome (BLS) [MIM:241850]. BLS is associated with thyroid agenesis, cleft palate and choanal atresia.
  • 配列類似性
    Contains 1 fork-head DNA-binding domain.
  • 翻訳後修飾
    Phosphorylated.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKH L15 antibody
    • FKHL 15 antibody
    • FKHL15 antibody
    • Forkhead (Drosophila) like 15 antibody
    • Forkhead box E1 antibody
    • Forkhead box E2 antibody
    • Forkhead box protein E1 antibody
    • Forkhead box protein E2 antibody
    • Forkhead drosophila homolog like 15 antibody
    • Forkhead like 15 antibody
    • Forkhead related protein FKHL 15 antibody
    • Forkhead related protein FKHL15 antibody
    • Forkhead-related protein FKHL15 antibody
    • FOX E1 antibody
    • FOX E2 antibody
    • FOXE 1 antibody
    • FOXE 2 antibody
    • FOXE1 antibody
    • FOXE1_HUMAN antibody
    • FOXE2 antibody
    • HFKH 4 antibody
    • HFKH4 antibody
    • HFKL 5 antibody
    • HFKL5 antibody
    • HNF-3/fork head-like protein 5 antibody
    • Thyroid transcription factor 2 antibody
    • TITF 2 antibody
    • TITF2 antibody
    • TTF 2 antibody
    • TTF-2 antibody
    • TTF2 antibody
    see all

Anti-FOXE1 antibody 画像

  • ab5080 (2µg/ml) staining of paraffin embedded Human Thyroid Gland staining in thyrocytes. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining

  • ab5080 (2.5 µg/ml) staining FOXE1 in paraffin-embedded sections of human testis. Steamed antigen retrieval with citrate buffer (pH 6) was performed.

プロトコール

Anti-FOXE1 antibody (ab5080) 使用論文

This product has been referenced in:
  • Bychkov A  et al. Patterns of FOXE1 expression in papillary thyroid carcinoma by immunohistochemistry. Thyroid 23:817-28 (2013). Read more (PubMed: 23327367) »
  • Bullock M  et al. Association of FOXE1 polyalanine repeat region with papillary thyroid cancer. J Clin Endocrinol Metab 97:E1814-9 (2012). EMSA . Read more (PubMed: 22736773) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (thyroid)
Specification
thyroid
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - 0.1% triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: TA°C
Username

Dr. Aurore Simon-Carré

Verified customer

投稿 May 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録