製品の概要

  • 製品名Anti-FOXD3 antibody
    FOXD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FOXD3
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    A synthesized peptide derived from an internal region of human FOXD3.

  • ポジティブ・コントロール
    • Human heart tissue and extracts from HUVEC and 293 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ELISA
  • 追加情報ELISA: 1/10000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
    • 組織特異性Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
    • 関連疾患Defects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis).
    • 配列類似性Contains 1 fork-head DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AIS1 antibody
      • forkhead box D3 antibody
      • Forkhead box protein D3 antibody
      • foxd3 antibody
      • FOXD3_HUMAN antibody
      • Genesis antibody
      • HFH2 antibody
      • HNF3/FH transcription factor genesis antibody
      • VAMAS2 antibody
      see all

    Anti-FOXD3 antibody 画像

    • All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilution

      Lane 1 : Extracts of HUVEC cells
      Lane 2 : Extracts of 293 cells
      Lane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 48 kDa
      Observed band size : 48 kDa
    • Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64807 at a 1:50 dilution.
      Left image untreated.
      Right image treated with immunising peptide.

    Anti-FOXD3 antibody (ab64807) 使用論文

    This product has been referenced in:
    • Douglas GC  et al. Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development. Endocr Rev 30:228-40 (2009). Read more (PubMed: 19299251) »

    See 1 Publication for this product

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    Thank you for your inquiry.

    I contacted the producing labs of these two antibodies. Although the immunogen sequences are confidential, they did an alignment with the sequence you provided.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"