Anti-FOXD3 抗体 (ab64807)
Key features and details
- Rabbit polyclonal to FOXD3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXD3 antibody
FOXD3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXD3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
A synthesized peptide derived from an internal region of human FOXD3.
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ポジティブ・コントロール
- Human heart tissue and extracts from HUVEC and 293 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64807の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ターゲット情報
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機能
Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. -
組織特異性
Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined. -
関連疾患
Defects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis). -
配列類似性
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 27022 Human
- Entrez Gene: 15221 Mouse
- Omim: 611539 Human
- SwissProt: Q9UJU5 Human
- SwissProt: Q61060 Mouse
- Unigene: 546573 Human
- Unigene: 4758 Mouse
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別名
- AIS1 antibody
- forkhead box D3 antibody
- Forkhead box protein D3 antibody
see all
画像
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All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilution
Lane 1 : Extracts of HUVEC cells
Lane 2 : Extracts of 293 cells
Lane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64807 at a 1:50 dilution.
Left image untreated.Right image treated with immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab64807 は 4 報の論文で使用されています。
- Liu R et al. FOXD3 and GAB2 as a pair of rivals antagonistically control hepatocellular carcinogenesis. FEBS J 289:4536-4548 (2022). PubMed: 35178865
- Cheng Y et al. FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2. J Cancer 12:6145-6154 (2021). PubMed: 34539887
- Huang J & Chen L IL-1β inhibits osteogenesis of human bone marrow-derived mesenchymal stem cells by activating FoxD3/microRNA-496 to repress wnt signaling. Genesis 55:N/A (2017). PubMed: 28509407
- Douglas GC et al. Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development. Endocr Rev 30:228-40 (2009). PubMed: 19299251