製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FOXD2
  • 特異性
    This antibody recognizes FOXD2
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide: GELPARSGPR APRDVLPHGH EPPAEEAEAD LAEDEEESGG CSDGEPRALA, corresponding to a region within N terminal amino acids 37-86 of Human FOXD2

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:62500.

WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Lane 1 : Marker
    Lane 2 : Anti-FOXD2 antibody (ab49128) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab49128 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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