製品の概要

  • 製品名Anti-FOXC2 antibody
    FOXC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FOXC2
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Horse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human FOXC2.

    (Peptide available as ab86226.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Whole Cell Lysates: Jurkat, MCF7, MDA-MB-361, A549, PC12.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.
    • 関連疾患Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
      Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
      Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
    • 配列類似性Contains 1 fork-head DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Drosphilia Forkhead Homolog Like 14 antibody
      • FKHL 14 antibody
      • FKHL14 antibody
      • Forkhead Box C2 antibody
      • Forkhead box protein C2 antibody
      • Forkhead related protein FKHL14 antibody
      • Forkhead-related protein FKHL14 antibody
      • FOX C2 antibody
      • Foxc2 antibody
      • FOXC2_HUMAN antibody
      • LD antibody
      • Mesenchyme fork head protein 1 antibody
      • Mesenchyme Forkhead 1 antibody
      • MFH 1 antibody
      • MFH 1 protein antibody
      • MFH-1 protein antibody
      • MFH1 antibody
      • Transcription factor FKH 14 antibody
      • Transcription factor FKH-14 antibody
      see all

    Anti-FOXC2 antibody 画像

    • All lanes : Anti-FOXC2 antibody (ab65141) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 54 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • ab65141 staining FOXC2 in mouse bone marrow tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocking was performed (5 minutes of peroxidase block and 10 minutes of protein block) for 15 minutes at 20°C. The primary antibody was diluted at 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

      See Abreview

    Anti-FOXC2 antibody (ab65141) 使用論文

    This product has been referenced in:
    • Pan J  et al. Cyclooxygenase-2 induced ß1-integrin expression in NSCLC and promoted cell invasion via the EP1/MAPK/E2F-1/FoxC2 signal pathway. Sci Rep 6:33823 (2016). Read more (PubMed: 27654511) »
    • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (embryo head/periocular region)
    Permeabilization Yes - triton 0,5%
    Specification embryo head/periocular region
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Nov 17 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Heart)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
    Permeabilization Yes - 0.025% Triton x-100
    Specification Heart
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

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    Verified customer

    投稿 Oct 16 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (bone marrow)
    Specification bone marrow
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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    Mr. Antibody Solutions

    Verified customer

    投稿 Jan 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"