Anti-FOXC2 抗体 (ab55004)

製品の概要

  • 製品名Anti-FOXC2 antibody
    FOXC2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to FOXC2
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant fragment: AASWYLNHSG DLNHLPGHTF AAQQQTFPNV REMFNSHRLG IENSTLGESQ VSGNASCQLP YRSTPPLYRH AAPYSYDCTK Y, corresponding to amino acids 421-502 of Human FOXC2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.
  • 関連疾患Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
    Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
    Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Drosphilia Forkhead Homolog Like 14 antibody
    • FKHL 14 antibody
    • FKHL14 antibody
    • Forkhead Box C2 antibody
    • Forkhead box protein C2 antibody
    • Forkhead related protein FKHL14 antibody
    • Forkhead-related protein FKHL14 antibody
    • FOX C2 antibody
    • Foxc2 antibody
    • FOXC2_HUMAN antibody
    • LD antibody
    • Mesenchyme fork head protein 1 antibody
    • Mesenchyme Forkhead 1 antibody
    • MFH 1 antibody
    • MFH 1 protein antibody
    • MFH-1 protein antibody
    • MFH1 antibody
    • Transcription factor FKH 14 antibody
    • Transcription factor FKH-14 antibody
    see all

Anti-FOXC2 antibody 画像

  • FOXC2 antibody (ab55004) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lateral ventricle wall.


  • Predicted band size : 54 kDa
    FOXC2 antibody (ab55004) at 1ug/lane + PC-12 cell lysate at 25ug/lane.
  • ab55004 at 10 ug/ml staining FOXC2 in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing HEK293 cells stained with ab55004 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55004, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-FOXC2 antibody (ab55004) 使用論文

ab55004 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (any kD gel)
Sample Human Tissue lysate - whole (human nasal tissue)
Specification human nasal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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Mr. Michael Könnecke

Verified customer

投稿 Aug 06 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I have issued a free of charge replacement with the order number *** for the goat polyclonal anti...

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Thank you for contacting us about the issue you are having with IHC and this antibody. The protocol you are using, with heat-mediated antigen retrieval in citrate buffer, is correct and you should see a stain for FOXC2 with this procedure. Are you conf...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tumor)
Specification tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
Username

Abcam user community

Verified customer

投稿 Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"