製品の概要

  • 製品名Anti-FOXA3 antibody
    FOXA3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FOXA3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human FOXA3 aa 250 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P55318

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Liver tissue lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

  • 機能Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis.
  • 組織特異性Expressed in erythroleukemia and hepatoma cell lines and in liver and pancreas. Not expressed in any other cell lines or tissues examined.
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 発生段階Detected in prenatal liver nuclear extracts (12.4-27 weeks estimated gestational age). Not detected in postnatal liver samples.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKHH3 antibody
    • Fork head-related protein FKH H3 antibody
    • forkhead box A3 antibody
    • Forkhead box protein A3 antibody
    • Foxa3 antibody
    • FOXA3_HUMAN antibody
    • hepatic nuclear factor-3-beta antibody
    • hepatocyte nuclear factor 3 antibody
    • hepatocyte nuclear factor 3 gamma antibody
    • Hepatocyte nuclear factor 3-gamma antibody
    • HNF-3-gamma antibody
    • HNF-3G antibody
    • HNF3B antibody
    • HNF3G antibody
    • TCF-3G antibody
    • TCF3G antibody
    • Transcription factor 3G antibody
    see all

Anti-FOXA3 antibody 画像

  • Anti-FOXA3 antibody (ab108454) at 1 µg/ml (Milk blocking 3%) + Liver (Human) Tissue Lysate - adult normal tissue at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 34 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab108454 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-FOXA3 antibody (ab108454) 使用論文

ab108454 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"