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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab108422 as a replacement.
Our Abpromise guarantee covers the use of ab60721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent concentration. PubMed: 20650961|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).|
|ELISA||Use at an assay dependent concentration. The detection limit for recombinant tagged FOXA2 is approximately 0.03ng/ml as a capture antibody.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Immunoprecipitation analysis of FOXA2 transfected lysate using ab60721 and protein A magnetic bead
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"