製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to FOXA2
  • 特異性
    This antibody is expected to recognise both reported isoforms.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide: GVYSRPIMNSS, corresponding to amino acids 447-457 of Human FOXA 2.

  • ポジティブ・コントロール
    • Human liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF 1/50. PubMed: 19564924
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FOXA2 peptide (ab23065). A nuclear extraction procedure may be required to concentrate the protein in samples.

ターゲット情報

  • 機能
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • 配列類似性
    Contains 1 fork-head DNA-binding domain.
  • 翻訳後修飾
    Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • 細胞内局在
    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Forkhead box A2 antibody
    • Forkhead box protein A2 antibody
    • FOX A2 antibody
    • foxa2 antibody
    • FOXA2_HUMAN antibody
    • Hepatic nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3 antibody
    • Hepatocyte nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3-beta antibody
    • HNF 3B antibody
    • HNF-3-beta antibody
    • HNF-3B antibody
    • HNF3B antibody
    • MGC19807 antibody
    • TCF 3B antibody
    • TCF-3B antibody
    • TCF3B antibody
    • Transcription factor 3B antibody
    see all

Anti-FOXA2 antibody 画像

  • Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Human Liver lysate at 1 µg/ml

    Predicted band size : 48 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-FOXA2 antibody (ab5074) 使用論文

This product has been referenced in:
  • Bhanu NV  et al. Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation. Proteomics 16:448-58 (2016). Flow Cyt ; Human . Read more (PubMed: 26631989) »
  • Saitoh I  et al. Choice of Feeders Is Important When First Establishing iPSCs Derived From Primarily Cultured Human Deciduous Tooth Dental Pulp Cells. Cell Med 8:9-23 (2015). Read more (PubMed: 26858904) »

See all 9 Publications for this product

Product Wall

Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Xenopus laevis Tissue lysate - whole (whole embryo)
Specification
whole embryo
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Apr 10 2015

Vielen Dank für Ihren Anruf.

Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. This antibody is yet to be tested in mouse cells. However, I would predict a favourable result given that the immunogen used to raise this anti...

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I'm sorry to hear you are having a problem with ab5074. This antibody has not been tested in HepG2 cells however it has been tested in human liver lysate and as HepG2 cells are liver derived I would suspect they express good levels of FOxa2. Howeve...

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Thank you for your enquiry and your interest in our products. Ab5074 recognizes Foxa2 which is a transcription activator for a number of liver genes. As the legend of the Western image on the on-line datasheet shows this antibody has been tested for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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