製品の概要

  • 製品名
    Anti-FOXA1 antibody [EP4131]
    FOXA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP4131] to FOXA1
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human FOXA1.

  • ポジティブ・コントロール
    • HepG2, PC-3, SW480, and Human colon cancer lysates
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能
      Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
    • 組織特異性
      Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
    • 配列類似性
      Contains 1 fork-head DNA-binding domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • forkhead box A1 antibody
      • Forkhead box protein A1 antibody
      • FOX A1 antibody
      • FOXA1 antibody
      • FOXA1_HUMAN antibody
      • hepatocyte nuclear factor 3 alpha antibody
      • Hepatocyte nuclear factor 3-alpha antibody
      • HNF 3A antibody
      • HNF-3-alpha antibody
      • HNF-3A antibody
      • HNF3A antibody
      • MGC33105 antibody
      • TCF 3A antibody
      • TCF-3A antibody
      • TCF3A antibody
      • Transcription factor 3A antibody
      see all

    画像

    • All lanes : Anti-FOXA1 antibody [EP4131] (ab109760) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : PC-3 lysate
      Lane 3 : SW480 lysate
      Lane 4 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 49 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Tu H  et al. MicroRNA-212 inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting FOXA1. Onco Targets Ther 8:2227-35 (2015). Read more (PubMed: 26347321) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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