製品の概要

  • 製品名Anti-FOXA1 antibody [3C1]
    FOXA1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3C1] to FOXA1
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 367-473 of Human FOXA1 with Propietary tag (NP_004487).

  • ポジティブ・コントロール
    • HepG2 and MCF7 cell lysates; Human Prostate tissue; HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • 組織特異性Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • forkhead box A1 antibody
    • Forkhead box protein A1 antibody
    • FOX A1 antibody
    • FOXA1 antibody
    • FOXA1_HUMAN antibody
    • hepatocyte nuclear factor 3 alpha antibody
    • Hepatocyte nuclear factor 3-alpha antibody
    • HNF 3A antibody
    • HNF-3-alpha antibody
    • HNF-3A antibody
    • HNF3A antibody
    • MGC33105 antibody
    • TCF 3A antibody
    • TCF-3A antibody
    • TCF3A antibody
    • Transcription factor 3A antibody
    see all

Anti-FOXA1 antibody [3C1] 画像

  • Anti-FOXA1 antibody [3C1] (ab125091) at 1/500 dilution + HepG2 cell lysate

    Predicted band size : 49 kDa
  • Anti-FOXA1 antibody [3C1] (ab125091) at 1/500 dilution + MCF7 cell lysate

    Predicted band size : 49 kDa
  • ab125091, at 5 µg/ml, staining FOXA1 in Formalin-fixed, Paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab125091 at 10µg/ml dilution staining FOXA1 in HeLa cells by Immunofluorescence.

Anti-FOXA1 antibody [3C1] (ab125091) 使用論文

This product has been referenced in:
  • Simeonov KP & Uppal H Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs. PLoS One 9:e100134 (2014). ICC/IF ; Human . Read more (PubMed: 24963715) »

See 1 Publication for this product

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