製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to Fox2 / RBM9
  • アプリケーション
    適用あり: WB, IHC-P, ICC, Flow Cyt, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: MEKKKMVTQG NQEPTTTPDA MVQPFTTIPF PPPPQNGIPT EYGVPHTQDY AGQTGEHNLT LYGSTQAHGE QSSNSPSTQN GSLTTEGGAQ TDGQQSQTQS , corresponding to amino acids 1-100 of Human Fox2/ RBM9

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.
  • 配列類似性
    Contains 1 RRM (RNA recognition motif) domain.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HRNBP2 antibody
    • Fox 1 homologue antibody
    • Fox-1 homolog B antibody
    • FOX2 antibody
    • FXH antibody
    • Hexaribonucleotide-binding protein 2 antibody
    • HNRBP2 antibody
    • RBFOX2 antibody
    • Repressor of tamoxifen transcriptional activity antibody
    • RFOX2_HUMAN antibody
    • RNA binding motif protein 9 antibody
    • RNA binding protein 9 antibody
    • RNA binding protein fox-1 homolog 2 antibody
    • RNA-binding motif protein 9 antibody
    • RNA-binding protein 9 antibody
    • RTA antibody
    see all

画像

  • Fox2 / RBM9 antibody (ab57154) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.


  • Predicted band size : 41 kDa
    Fox2 / RBM9 antibody (ab57154) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • ICC/IF image of ab57154 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57154, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab57154 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57154, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献

This product has been referenced in:
  • Verma SK  et al. Rbfox2 function in RNA metabolism is impaired in hypoplastic left heart syndrome patient hearts. Sci Rep 6:30896 (2016). Read more (PubMed: 27485310) »
  • Cammas A  et al. hnRNP A1-mediated translational regulation of the G quadruplex-containing RON receptor tyrosine kinase mRNA linked to tumor progression. Oncotarget 7:16793-805 (2016). IHC . Read more (PubMed: 26930004) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (fetal lung)
Loading amount
20 µg
Specification
fetal lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain coronal)
Specification
Brain coronal
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS + 0.1% Tween20
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Melanie White

Verified customer

投稿 Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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