Anti-Follistatin 抗体 (ab64490)
Key features and details
- Rabbit polyclonal to Follistatin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Follistatin antibody
Follistatin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Follistatin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Follistatin.
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ポジティブ・コントロール
- Recombinant human Follistatin protein (ab50163) can be used as a positive control in WB. This antibody gave a positive signal in the following Lysates: Mouse Liver Tissue, Raji Whole Cell, MOLT4 Whole Cell, Mouse Colon Tissue, Rat Liver Tissue, Human liver tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64490の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
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機能
Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). -
組織特異性
Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid. -
配列類似性
Contains 3 follistatin-like domains.
Contains 3 Kazal-like domains.
Contains 1 TB (TGF-beta binding) domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 396119 Chicken
- Entrez Gene: 327681 Cow
- Entrez Gene: 10468 Human
- Entrez Gene: 14313 Mouse
- Entrez Gene: 445002 Pig
- Entrez Gene: 24373 Rat
- Omim: 136470 Human
- SwissProt: Q90844 Chicken
see all -
別名
- Activin binding protein antibody
- Activin-binding protein antibody
- Follistatin antibody
see all
画像
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All lanes : Anti-Follistatin antibody (ab64490) at 1 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 38 kDa
Additional bands at: 15 kDa (possible non-specific binding), 35 kDa (possible isoform), 38 kDa (possible isoform), 60 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 2 minutesBlocking buffer: 2% BSA
Gel type: MOPS
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All lanes : Anti-Follistatin antibody (ab64490) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 5 : Colon (Mouse) Tissue Lysate
Lane 6 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 15 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab64490 は 6 報の論文で使用されています。
- Reichel C et al. Detection of black market follistatin 344. Drug Test Anal 12:1522-1533 (2020). PubMed: 33460286
- Liu N et al. Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling. PLoS Genet 15:e1008368 (2019). PubMed: 31518356
- Reichel C et al. Detection of black market follistatin 344. Drug Test Anal 11:1675-1697 (2019). PubMed: 31758732
- Singh R et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). PubMed: 29618935
- Fitzgerald AM et al. The effects of transforming growth factor-ß2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues. Invest Ophthalmol Vis Sci 53:7358-69 (2012). WB . PubMed: 23010638
- Garcia LA et al. 1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 152:2976-86 (2011). WB ; Mouse . PubMed: 21673099