Rabbit polyclonal to Follistatin
Mouse, Rat, Human
Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Follistatin.
(Peptide available as
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.
Contains 3 follistatin-like domains.
Contains 3 Kazal-like domains. Contains 1 TB (TGF-beta binding) domain.
Information by UniProt
Activin binding protein antibody
Activin-binding protein antibody
Western blot - Follistatin antibody (ab64490)
All lanes : Anti-Follistatin antibody (ab64490) at 1 µg/ml Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 2 : Liver (Mouse) Tissue Lysate Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lane 5 : Colon (Mouse) Tissue Lysate Lane 6 : Liver (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 38 kDa Observed band size : 38 kDa Additional bands at : 15 kDa,75 kDa. We are unsure as to the identity of these extra bands. Exposure time : 1 minute
This product has been referenced in:
et al. The effects of transforming growth factor-ß2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues. Invest Ophthalmol Vis Sci 53:7358-69 (2012).
Read more (PubMed: 23010638) »
et al. 1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 152:2976-86 (2011).
Read more (PubMed: 21673099) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"