製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FNIP1
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A peptide at the N-terminus of FNIP1, MAPTLFQKLFSKRTGLGAC-amide

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 131 kDa.
IP Use at an assay dependent dilution.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 関連性
      May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
    • 細胞内局在
      Cytoplasm. Note: Co-localizes with FLCN in the cytoplasm.
    • 参照データベース
    • 別名
      • KIAA1961 antibody
      • FLCN-interacting protein antibody
      • FNIP1 antibody
      • Folliculin interacting protein 1 antibody

    参考文献

    This product has been referenced in:
    • Siggs OM  et al. Mutation of Fnip1 is associated with B-cell deficiency, cardiomyopathy, and elevated AMPK activity. Proc Natl Acad Sci U S A 113:E3706-15 (2016). Read more (PubMed: 27303042) »
    • Liu J  et al. Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit. EMBO Mol Med 8:1212-1228 (2016). Read more (PubMed: 27506764) »

    See all 2 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK 293)
    Loading amount
    25 µg
    Specification
    HEK 293
    Gel Running Conditions
    Non-reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

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    Verified customer

    投稿 Aug 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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