製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FMNL1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 631 - 873 of Human FMNL1 (NP_005883).

  • ポジティブ・コントロール
    • 293T, A431, HepG2, MOLT4 and Raji cell lysates. H1299 and HeLa whole cell lysate.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 122 kDa.

ターゲット情報

  • 機能
    May play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
  • 組織特異性
    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 配列類似性
    Belongs to the formin homology family.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • ドメイン
    The DAD domain regulates activation via by an autoinhibitory interaction with the N-terminus. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
  • 翻訳後修飾
    Myristoylation mediates membrane localization and blebbing.
  • 細胞内局在
    Cytoplasm > cell cortex. Cell projection > bleb. Colocalized with F-actin in bleb protrusions and Cytoplasm. Cell membrane. Cytoplasmic vesicle > phagosome. Recruited to actin-rich phagosomes during phagocytosis. Translocates to the plasma membrane upon activation by RAC1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 8030453N10Rik antibody
    • AI553564 antibody
    • C17orf1 antibody
    • C17orf1B antibody
    • CLL associated antigen KW 13 antibody
    • CLL-associated antigen KW-13 antibody
    • FHOD4 antibody
    • FMNL antibody
    • FMNL_HUMAN antibody
    • Fmnl1 antibody
    • Fnrl antibody
    • Formin like 1 antibody
    • Formin like antibody
    • Formin related protein antibody
    • Formin-like protein 1 antibody
    • Frls antibody
    • KW 13 antibody
    • KW13 antibody
    • Leukocyte formin antibody
    • MGC133052 antibody
    • MGC1894 antibody
    • MGC21878 antibody
    see all

Anti-FMNL1 antibody 画像

  • All lanes : Anti-FMNL1 antibody (ab97456) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 122 kDa
    7.5% SDS PAGE

プロトコール

Anti-FMNL1 antibody (ab97456) 使用論文

This product has been referenced in:
  • Monzo P  et al. Mechanical confinement triggers glioma linear migration dependent on formin FHOD3. Mol Biol Cell 27:1246-61 (2016). Rat . Read more (PubMed: 26912794) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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