特記事項（精製）ab54361 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 122 kDa.
Use at an assay dependent dilution.
機能May play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
組織特異性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
配列類似性Belongs to the formin homology family. Contains 1 DAD (diaphanous autoregulatory) domain. Contains 1 FH2 (formin homology 2) domain. Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
ドメインThe DAD domain regulates activation via by an autoinhibitory interaction with the N-terminus. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
翻訳後修飾Myristoylation mediates membrane localization and blebbing.
細胞内局在Cytoplasm > cell cortex. Cell projection > bleb. Colocalized with F-actin in bleb protrusions and Cytoplasm. Cell membrane. Cytoplasmic vesicle > phagosome. Recruited to actin-rich phagosomes during phagocytosis. Translocates to the plasma membrane upon activation by RAC1.