製品名 Anti-FMN1 antibody
Mouse polyclonal to FMN1
特異性 This antibody is reactive against mammalian transfected lysate.
WB, ICC/IF more details
FMN1 (AAI03693.1, 1 a.a. ~ 503 a.a) full-length human protein.
FMN1 transfected 293T cell lysate
保存方法 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファー Preservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
精製度 Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 85 kDa.
Use at an assay dependent concentration.
関連性 FMN1 plays a role in the formation of adherens junction and the polymerization of linear actin cables. Interacts with alpha-catenin and may interact with tubulin
細胞内局在 Nucleus. Cytoplasm. Cell junction, adherens junction; Peripheral membrane protein; Cytoplasmic side.
Formin (limb deformity) antibody
Formin 1 antibody
Anti-FMN1 antibody 画像
Western blot - FMN1 antibody (ab68058)
All lanes : Anti-FMN1 antibody (ab68058) at 1/500 dilution Lane 1 : FMN1 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Observed band size : 85 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - FMN1 antibody (ab68058) Image courtesy of an anonymous Abreview
ab68058 staining FMN1 in mouse embryonic fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed using 4% paraformaldehyde, permeabilised in 1% Triton X-100 and then blocked using 10% horse serum + 5% BSA for 1 hour at room temperature. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"