Anti-Fluorescein 抗体 (Texas Red) (ab6654)


  • 製品名Anti-Fluorescein antibody (Texas Red)
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Fluorescein (Texas Red)
  • 標識Texas Red ®. Ex: 596nm, Em: 620nm
  • アプリケーション適用あり: ELISA, Flow Cyt, WB, ICC/IFmore details
  • 免疫原

    Fluorescein conjugated to Goat IgG

  • 特記事項

    Texas RedTM is a registered trademark of Molecular Probes Inc.

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.9 moles Texas RedTM per mole of Goat IgG.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab6654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • 関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (Texas Red) (ab6654) 使用論文

This product has been referenced in:
  • Kang HM  et al. Insulin-secreting cells from human eyelid-derived stem cells alleviate type I diabetes in immunocompetent mice. Stem Cells 27:1999-2008 (2009). IHC-P . Read more (PubMed: 19544420) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Zebrafish Cell (heart)
Specification heart
Permeabilization Yes - proteinase K
Fixative Formaldehyde

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投稿 Jul 12 2013

The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 1-4:1