Anti-Fluorescein 抗体 (Rhodamine) (ab7253)

製品の概要

  • 製品名Anti-Fluorescein antibody (Rhodamine)
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Fluorescein (Rhodamine)
  • 標識Rhodamine. Ex: 550nm, Em: 570nm
  • アプリケーション適用あり: Immunomicroscopy, Flow Cytmore details
  • 免疫原

    Fluorescein conjugated to Goat IgG

  • 特記事項

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Goat IgG.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy
Flow Cyt
  • 追加情報Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  • ターゲット情報

    • 関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
    • 別名
      • FI antibody
      • FITC antibody
      • fluorescein isothyocianate antibody
      • fluorescyl antibody

    Anti-Fluorescein antibody (Rhodamine) (ab7253) 使用論文

    ab7253 has not yet been referenced specifically in any publications.

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    The antibody has not been tested for binding to free flourescein, but some binding to the free form is expected since the antibody is polyclonal. The antibody is tested against flourescein conjugated protein. I hea...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"