製品の概要

  • 製品名Anti-Fluorescein antibody (HRP)
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Fluorescein (HRP)
  • 標識HRP
  • 特異性This antibody reacts with FITC-conjugated proteins.
  • アプリケーション適用あり: ELISA, IHC-P, IHC-Fr, WBmore details
  • 免疫原

    Fluorescein conjugated to Goat IgG

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.2
    Preservative: 0.1% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/200000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/5000.

ターゲット情報

  • 関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (HRP) (ab6656) 使用論文

This product has been referenced in:
  • Shigdar S  et al. The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One 8:e57613 (2013). IHC-P ; Human . Read more (PubMed: 23460885) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (thymus)
Antigen retrieval step None
Permeabilization Yes - Tween20
Specification thymus
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 29 2016

Thank you for your inquiry and support of our products. The antibody is only tested and guaranteed against derivatives of FITC (Fluorescein isothiocyanate) but it may react with other forms as well. It has not been tested against 6’-FAM. The ant...

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Thank you for contacting us.

Without a direct comparison, it is a little difficult to choose between the two. The ab6656 datasheet also has a review of IHC with TSA amplification on frozen tissue, after applying the antibody at a dilution of...

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Thank you for your enquiry. The concentration of this antibody is 1.000 mg/ml, however the working dilution/concentration needs to be determined since the signal depends on several factors (such as expression levels of the target, sample preparatio...

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Thank you for your phone call today and for your patience while I was in contact with the lab. The lab confirmed that ab6656 has been tested on FITC-conjugated proteins, and so the antibody is appropriate for your studies. I hope this informatio...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse mammary carcinoma metastasis)
Specification mouse mammary carcinoma metastasis
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 01 2009

Thank you for your enquiry. Yes, I would consider it highly likely that you will be able to use an anti-fluorescein antibody to immunoprecipitate cross linked chromatin associated with your small molecule. You may also wish to consider NChIP as thi...

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Thank you for your enquiry. Yes, the chromogen AEC kit is used after using the HRP conjugated fluorescein antibody in immunohistochemical techniques. If you are doing ELISA then a TMB kit is better.

Sorry, we do not have any data on the specific activity of the HRP.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"