製品の概要

  • 製品名Anti-Fluorescein antibody (HRP)
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fluorescein (HRP)
  • 標識HRP
  • 特異性Specific to Fluoresceine.
  • アプリケーション適用あり: IP, IHC-P, WB, ELISAmore details
  • 種交差性
    No reactivity with immunoglobulin of any species.
  • 免疫原

    Fluorescein coupled to KLH

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA
  • Concentration information loading...
  • 特記事項(精製)Solid phase hapten antibody capture. Affinity purified and absorbed antibody covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated substitution ratio: HRP:IgG is >1.5
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 19706527
IHC-P Use at an assay dependent concentration.
WB 1/20 - 1/200.
ELISA 1/200 - 1/1000.

ターゲット情報

  • 関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (HRP) 画像

  • Immunohistochemical analysis of chicken trachea tissue, infected with an avian influenza virus, staining Fluorescein with ab19492.
    Fluoroscein-labeled influenza viruses were incubated with tissue overnight at 4°C. Sections were incubated with primary antibody and the signal was amplified with a tyramide signal amplification system.

Anti-Fluorescein antibody (HRP) (ab19492) 使用論文

This product has been referenced in:
  • Kraaij MD  et al. The NOX2-mediated ROS producing capacity of recipient cells is associated with reduced T cell infiltrate in an experimental model of chronic renal allograft inflammation. Transpl Immunol 30:65-70 (2014). Read more (PubMed: 24365514) »
  • Jourdain E  et al. The Pattern of Influenza Virus Attachment Varies among Wild Bird Species. PLoS One 6:e24155 (2011). IHC-P . Read more (PubMed: 21909418) »

See all 4 Publications for this product

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Without a direct comparison, it is a little difficult to choose between the two. The ab6656 datasheet also has a review of IHC with TSA amplification on frozen tissue, after applying the antibody at a dilution of...

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