Anti-Fluorescein 抗体 [BT.6A4] (Biotin) (ab6215)

製品の概要

  • 製品名Anti-Fluorescein antibody [BT.6A4] (Biotin)
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BT.6A4] to Fluorescein (Biotin)
  • 標識Biotin
  • 特異性The antibody reacts with free and bound fluorescein.
  • アプリケーション適用あり: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 特記事項(精製)Purified anti-fluorescein biotin conjugate.
  • ポリ/モノモノクローナル
  • クローン名BT.6A4
  • ミエローマunknown
  • アイソタイプIgM
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000.
IHC-Fr 1/200 - 1/2000.
In situ hybridization Use at an assay dependent dilution.

ターゲット情報

  • 関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody [BT.6A4] (Biotin) (ab6215) 使用論文

ab6215 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"