Anti-Fluorescein 抗体 (Alkaline Phosphatase) (ab6657)

製品の概要

  • 製品名Anti-Fluorescein antibody (Alkaline Phosphatase)
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Fluorescein (Alkaline Phosphatase)
  • 標識Alkaline Phosphatase
  • アプリケーション適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
  • 免疫原

    Fluorescein conjugated to Goat IgG

  • 特記事項

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/1000 - 1/5000.
ELISA 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
WB 1/1000 - 1/5000.

ターゲット情報

  • 関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (Alkaline Phosphatase) (ab6657) 使用論文

ab6657 has not yet been referenced specifically in any publications.

Product Wall

Based on the Lot #6189 of ab6657, a working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product (assayed against 1.0 ug of Fluorescein conjugated IgG in a standard capture ELISA using pNPP p-nitrophenyl phosp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"