製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Fluorescein
  • 特異性
    Specific to Fluoresceine. No cross reactivity with immunoglobulin of any species.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 免疫原

    Fluoresceine conjugated to KHL.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Immunoaffinity chromatography
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

プロトコール

参考文献

This product has been referenced in:
  • Koole C  et al. Genetically encoded photocross-linkers determine the biological binding site of exendin-4 peptide in the N-terminal domain of the intact human glucagon-like peptide-1 receptor (GLP-1R). J Biol Chem 292:7131-7144 (2017). Read more (PubMed: 28283573) »
  • Wakayama S  et al. Chemical labelling for visualizing native AMPA receptors in live neurons. Nat Commun 8:14850 (2017). Read more (PubMed: 28387242) »

See all 7 Publications for this product

レビューと Q&A

Thank you for your enquiry.

I am sorry to confirm that regrettablythese antibodies have not yet been tested and guaranteed in this type of ELISAcoating the antibody on theplate using protein A. They have been testedas detection antibodies in...

Read More

The reactivity of ab19491 has not been tested against FAM, this polyclonal antibody has been tested and will react with DTAF ( a fluorescein derivative) in addition to FITC.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録