製品の概要

  • 製品名Anti-Fluorescein antibody
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fluorescein
  • 特異性Specific to Fluoresceine. No cross reactivity with immunoglobulin of any species.
  • アプリケーション適用あり: ELISA, WBmore details
  • 免疫原

    Fluoresceine conjugated to KHL.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Immunoaffinity chromatography
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

ターゲット情報

  • 関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (ab19491) 使用論文

This product has been referenced in:
  • Valentin-Hansen L  et al. Mapping Substance P Binding Sites on the Neurokinin-1 Receptor Using Genetic Incorporation of a Photoreactive Amino Acid. J Biol Chem 289:18045-18054 (2014). WB . Read more (PubMed: 24831006) »
  • Ishizuka A  et al. Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1. Genes Cells 19:704-21 (2014). Read more (PubMed: 25145264) »

See all 3 Publications for this product

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Thank you for your enquiry.

I am sorry to confirm that regrettablythese antibodies have not yet been tested and guaranteed in this type of ELISAcoating the antibody on theplate using protein A. They have been testedas detection antibodies in...

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The reactivity of ab19491 has not been tested against FAM, this polyclonal antibody has been tested and will react with DTAF ( a fluorescein derivative) in addition to FITC.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"